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w magna c2c maky  (ATCC)


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    ATCC w magna c2c maky
    W Magna C2c Maky, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 6563 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/w+magna+c2c+maky/Hybridoma+murine+cells+3C6%2E4%2E2/pm32860684-39-5-9
    Average 97 stars, based on 6563 article reviews
    w magna c2c maky - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Isolation:

    Article Title: Impact of inter-amoebic phagocytosis on the L. pneumophila growth.
    Article Snippet: Free-living amoebae are known to act as replication niches for the pathogenic bacterium Legionella pneumophila in freshwater environments.. However, we previously reported that some strains of the Willaertia magna species are more resistant to L. pneumophila infection and differ in their ability to support its growth.. From this observation, we hypothesize that L. pneumophila growth in environment could be partly dependent on the composition of amoebic populations and on the possible interactions between different amoebic species.

    Filtration:

    Article Title: Impact of inter-amoebic phagocytosis on the L. pneumophila growth.
    Article Snippet: Free-living amoebae are known to act as replication niches for the pathogenic bacterium Legionella pneumophila in freshwater environments.. However, we previously reported that some strains of the Willaertia magna species are more resistant to L. pneumophila infection and differ in their ability to support its growth.. From this observation, we hypothesize that L. pneumophila growth in environment could be partly dependent on the composition of amoebic populations and on the possible interactions between different amoebic species.



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    Level of rpsL transcript in the different conditions, expressed as the number of copies in 1 × 10 6 L. pneumophila (Lp) ± SD and displayed using a log scale. Abbreviations: FREE: L. pneumophila strains alone; WILL: L. pneumophila strains cocultured with W. magna <t>C2c</t> Maky; ACANTH: L. pneumophila strains cocultured with A. castellanii ; T′0: reference transcript level; 3D: transcript level after 3 days. ANOVA 2: Factor 1: “ L. pneumophila strain”, p < 0.0001; Factor 2: “culture conditioned”, p < 0.0001; Interaction Factor 1 × Factor 2, p < 0.0001.
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    Summary of the <t> W. magna c2c </t> genome.
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    Level of rpsL transcript in the different conditions, expressed as the number of copies in 1 × 10 6 L. pneumophila (Lp) ± SD and displayed using a log scale. Abbreviations: FREE: L. pneumophila strains alone; WILL: L. pneumophila strains cocultured with W. magna C2c Maky; ACANTH: L. pneumophila strains cocultured with A. castellanii ; T′0: reference transcript level; 3D: transcript level after 3 days. ANOVA 2: Factor 1: “ L. pneumophila strain”, p < 0.0001; Factor 2: “culture conditioned”, p < 0.0001; Interaction Factor 1 × Factor 2, p < 0.0001.

    Journal: Pathogens

    Article Title: Mitigation of Expression of Virulence Genes in Legionella pneumophila Internalized in the Free-Living Amoeba Willaertia magna C2c Maky

    doi: 10.3390/pathogens9060447

    Figure Lengend Snippet: Level of rpsL transcript in the different conditions, expressed as the number of copies in 1 × 10 6 L. pneumophila (Lp) ± SD and displayed using a log scale. Abbreviations: FREE: L. pneumophila strains alone; WILL: L. pneumophila strains cocultured with W. magna C2c Maky; ACANTH: L. pneumophila strains cocultured with A. castellanii ; T′0: reference transcript level; 3D: transcript level after 3 days. ANOVA 2: Factor 1: “ L. pneumophila strain”, p < 0.0001; Factor 2: “culture conditioned”, p < 0.0001; Interaction Factor 1 × Factor 2, p < 0.0001.

    Article Snippet: The amoebae used in this study were W. magna C2c Maky ATCC PTA-7824 and A. castellanii ATCC 30010.

    Techniques:

    Measurement of the virulence index. ( A ) After 3 days in liquid medium; ( B ) after 3 days in liquid medium plus 3 days on buffered charcoal yeast extract (BCYE) plates. FREE: L. pneumophila strains alone; WILL: L. pneumophila strains cocultured with W. magna C2c Maky; ACANTH: L. pneumophila strains cocultured with A. castellanii ; T′0: reference virulence index; T3D: virulence index after 3 days; T6D: virulence index after 6 days, 3 days in liquid medium, and 3 days on BCYE plates.

    Journal: Pathogens

    Article Title: Mitigation of Expression of Virulence Genes in Legionella pneumophila Internalized in the Free-Living Amoeba Willaertia magna C2c Maky

    doi: 10.3390/pathogens9060447

    Figure Lengend Snippet: Measurement of the virulence index. ( A ) After 3 days in liquid medium; ( B ) after 3 days in liquid medium plus 3 days on buffered charcoal yeast extract (BCYE) plates. FREE: L. pneumophila strains alone; WILL: L. pneumophila strains cocultured with W. magna C2c Maky; ACANTH: L. pneumophila strains cocultured with A. castellanii ; T′0: reference virulence index; T3D: virulence index after 3 days; T6D: virulence index after 6 days, 3 days in liquid medium, and 3 days on BCYE plates.

    Article Snippet: The amoebae used in this study were W. magna C2c Maky ATCC PTA-7824 and A. castellanii ATCC 30010.

    Techniques:

    Summary of the W. magna c2c genome.

    Journal: Scientific Reports

    Article Title: Investigation of potential pathogenicity of Willaertia magna by investigating the transfer of bacteria pathogenicity genes into its genome

    doi: 10.1038/s41598-019-54580-6

    Figure Lengend Snippet: Summary of the W. magna c2c genome.

    Article Snippet: W. magna c2c maky cells (ATCC PTA-7824) were grown at 30 °C in 175 cm2 culture flasks (Thermo Fisher Scientific, Illkirch, France) containing 75 mL of SCGYEM medium .

    Techniques: Sequencing

    Comparison of the main genomic features of several amoebas.

    Journal: Scientific Reports

    Article Title: Investigation of potential pathogenicity of Willaertia magna by investigating the transfer of bacteria pathogenicity genes into its genome

    doi: 10.1038/s41598-019-54580-6

    Figure Lengend Snippet: Comparison of the main genomic features of several amoebas.

    Article Snippet: W. magna c2c maky cells (ATCC PTA-7824) were grown at 30 °C in 175 cm2 culture flasks (Thermo Fisher Scientific, Illkirch, France) containing 75 mL of SCGYEM medium .

    Techniques: Comparison

    Representation of horizontal transfer analysis. Phylogenetic trees for two W. magna c2c maky proteins of putative ARB origin. The trees were constructed using maximum-likelihood method based on the leucine rich repeat sequence of W. magna . The trees were performed with 18 ( a ) and 14 ( b ) homologous sequences of W. magna retrieved by BLASTp on NCBI. In red: W. magna c2c maky leucine rich repeat-containing protein ( a ) gene 14,561; ( b ) gene 11,963); in green: the ARB ( legionella geestiana ) homologs; in blue: homologs from legionella geestiana ( a ) or homologs from giant viruses ( b ); in black: homologs from other organisms.

    Journal: Scientific Reports

    Article Title: Investigation of potential pathogenicity of Willaertia magna by investigating the transfer of bacteria pathogenicity genes into its genome

    doi: 10.1038/s41598-019-54580-6

    Figure Lengend Snippet: Representation of horizontal transfer analysis. Phylogenetic trees for two W. magna c2c maky proteins of putative ARB origin. The trees were constructed using maximum-likelihood method based on the leucine rich repeat sequence of W. magna . The trees were performed with 18 ( a ) and 14 ( b ) homologous sequences of W. magna retrieved by BLASTp on NCBI. In red: W. magna c2c maky leucine rich repeat-containing protein ( a ) gene 14,561; ( b ) gene 11,963); in green: the ARB ( legionella geestiana ) homologs; in blue: homologs from legionella geestiana ( a ) or homologs from giant viruses ( b ); in black: homologs from other organisms.

    Article Snippet: W. magna c2c maky cells (ATCC PTA-7824) were grown at 30 °C in 175 cm2 culture flasks (Thermo Fisher Scientific, Illkirch, France) containing 75 mL of SCGYEM medium .

    Techniques: Construct, Sequencing

    Phylogenetic analysis of W. magna c2c maky Nf314 protein. The phylogenetic tree is based on protein Nf314 from W. magna c2c and other eukaryotic organisms including amoebas. Nf314 protein sequences of W. magna are not clustered with this homolog in N. fowleri . The protein Nf314 of W. magna c2c is indicated in green; that of non-pathogenic amoeba in blue and that of pathogenic amoeba ( N. fowleri ) in red.

    Journal: Scientific Reports

    Article Title: Investigation of potential pathogenicity of Willaertia magna by investigating the transfer of bacteria pathogenicity genes into its genome

    doi: 10.1038/s41598-019-54580-6

    Figure Lengend Snippet: Phylogenetic analysis of W. magna c2c maky Nf314 protein. The phylogenetic tree is based on protein Nf314 from W. magna c2c and other eukaryotic organisms including amoebas. Nf314 protein sequences of W. magna are not clustered with this homolog in N. fowleri . The protein Nf314 of W. magna c2c is indicated in green; that of non-pathogenic amoeba in blue and that of pathogenic amoeba ( N. fowleri ) in red.

    Article Snippet: W. magna c2c maky cells (ATCC PTA-7824) were grown at 30 °C in 175 cm2 culture flasks (Thermo Fisher Scientific, Illkirch, France) containing 75 mL of SCGYEM medium .

    Techniques: